首页|Synergistic effect of citral and carvacrol and their combination with mild heat against Cronobacter sakazakii CICC 21544 in reconstituted infant formula
Synergistic effect of citral and carvacrol and their combination with mild heat against Cronobacter sakazakii CICC 21544 in reconstituted infant formula
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NSTL
Elsevier
The combined effects of four substances, carvacrol, citral, thymol and caprylic acid, on the growth of Cronobacter sakazakii CICC 21544 were evaluated. Among the six combinations, only the combination of citral and carvacrol demonstrated a synergistic effect. This combination had a stronger growth inhibition effect than treatment with citral or carvacrol alone, which led to more portions of cells in the propidium iodide (PI) fluorescent region and caused more severe cell damage. Citral and carvacrol also showed promising and potent antibiofilm activity against C. sakazakii, which was evaluated using crystal violet assay, field emission scanning electron microscopy (FESEM) and qRT-PCR. Mild heat treatment combined with citral and carvacrol at a concentration of 2 or 3 MIC completely eradicated C. sakazakii in reconstituted infant formula. Moreover, the combination at 2 MIC had no significant effect on the color and aroma scores of reconstituted infant formulae. The combination of citral and carvacml could potentially be developed as an alternative or supplemental agent to reduce infections caused by C. sakazakii in reconstituted infant formula.
NSTL
Elsevier
