Cloning and characterization of squalene synthase gene from Poria cocos and its up-regulation by methyl jasmonate

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NSTL
Springer Nature

外文摘要：Squalene synthase (SQS) catalyzes the condensation of two molecules of farnesyl diphosphate to give presqualene diphosphate and the subsequent rearrangement to form squalene. The gene encoding squalene synthase was cloned from Poria cocos by degenerate PCR and inverse PCR. The open reading frame of the gene is 1,497 bp, which encodes 499 amino acid residues. A phylogenetic analysis revealed that P. cocos SQS belonged to the fungus group, and was more closely related to the SQS of Ganoderma lucidum than other fungi. The treatment of P. cocos with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of P. cocos sqs gene and the content of squalene in P. cocos. The transcriptional level of sqs gene was approximately fourfold higher than the control sample and the squalene content reached 128.62 mu g/g, when the concentration of MeJA was 300 mu M after 72 h induction.

外文关键词：

Poria cocosSqualene synthaseTriterpenoidsSqualeneMethyl jasmonate

作者：

Wen, Jia-Ming、Wang, Jian-Rong、Lin, Jun-Fang、Guo, Li-Qiong、You, Lin-Feng、Zeng, Xian-Lu

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作者单位：

Institute of Biomass Energy Research, South China Agricultural University, Guangzhou, 510642, Guangdong, China

Department of Bioengineering, College of Food Science, South China Agricultural University, 482 Wu-Shan Road, Tian-He District, Guangzhou, 510642, Guangdong, China

Guangdong VTR Bio-Tech Co., Ltd., Zhuhai, China

出版年：

2014

DOI：

10.1007/s11274-013-1477-z

World Journal of Microbiology & Biotechnology

SCI

ISSN：0959-3993

年,卷(期)：2014.30(2)

被引量11
参考文献量18