首页|Antifungal susceptibility of yeasts isolated from anthropogenic watershed
Antifungal susceptibility of yeasts isolated from anthropogenic watershed
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NSTL
Acad Brasileira De Ciencias
Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Diluvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Diluvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1-5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests showed a high prevalence of resistance to azole antifungals, where 16.8% of the isolates had a resistance profile to amphotericin B. The sequence analysis allowed the identification of Candida species, including potentially pathogenic species, and species of the Debaryomyces spp. The resistance to antifungals in yeasts isolated from Arroio Diluvio reinforces the importance of studies of environmental microbiota, and indicates that environmental degradation influences the phenotype displayed.
yeastantifungalwatershedresistance
van der Sand, Sueli T.、Milanezi, Ana C. M.、Witusk, Joao P. D.
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Univ Fed Rio Grande do Sul, Dept Microbiol Imunol & Parasitol, Inst Ciencias Basicas Saude, Rua Sarmento Leite 500, BR-90050170 Porto Alegre, RS, Brazil
NSTL
Acad Brasileira De Ciencias
