首页|Transcriptome analysis reveals decreased accumulation and toxicity of Cd in upland rice inoculated with arbuscular mycorrhizal fungi
Transcriptome analysis reveals decreased accumulation and toxicity of Cd in upland rice inoculated with arbuscular mycorrhizal fungi
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NSTL
Elsevier
? 2022 Elsevier B.V.There have been a few reports on the impacts of arbuscular mycorrhizal fungi (AMF) on the expression of genes associated with the uptake, transport and detoxification of Cd in upland rice, but there is no study on the impacts of AMF on the transcriptome of upland rice grown in Cd-contaminated soil. In the present work, a pot experiment was conducted to study the influences of the AMF-Glomus versiforme (Gv) on the Cd accumulation, photosynthesis, antioxidant enzymes and transcriptome in upland rice grown in soils supplemented with 10 μg Cd g?1 soil. Gv symbiosis evidently promoted the plant growth, P acquisition and photosynthetic characteristics in upland rice. In addition, 229 differentially expressed genes (DEGs) were identified in upland rice root inoculated with Gv, of which 149 DEGs were upregulated and 80 DEGs were downregulated. More importantly, one Cd transporter gene Nramp5 was significantly downregulated, thus reducing Cd absorption, transfer and accumulation in mycorrhizal upland rice. Moreover, four genes in the Gene Ontology (GO) term of cysteine biosynthesis and two metallothionein genes were upregulated, which could have decreased Cd phytotoxicity in upland rice inoculated with Gv. Meanwhile, one peroxidase gene, two genes in the GO term of peroxisome and one gene in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway of carotenoid biosynthesis were significantly upregulated, and enhanced the activities of catalase (CAT) and peroxidase (POD) in Gv-inoculated upland rice. Our results of transcriptome analysis initially elucidated the molecular mechanism by which AMF decreased the accumulation and toxicity of Cd in upland rice.
Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization Key Laboratory of Ecology and Environmental
NSTL
Elsevier
