首页|Ultrahigh-throughput screening of industrial enzyme-producing strains by droplet-based microfluidic system
Ultrahigh-throughput screening of industrial enzyme-producing strains by droplet-based microfluidic system
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NSTL
Oxford Univ Press
Droplet-based microfluidics has emerged as a powerful tool for single-cell screening with ultrahigh throughput, but its widespread application remains limited by the accessibility of a droplet microfluidic high-throughput screening (HTS) platform, especially to common laboratories having no background in microfluidics. Here, we first developed a microfluidic HTS platform based on fluorescence-activated droplet sorting technology. This platform allowed (i) encapsulation of single cells in monodisperse water-in-oil droplets; (ii) cell growth and protein production in droplets; and (iii) sorting of droplets based on their fluorescence intensities. To validate the platform, a model selection experiment of a binary mixture of Bacillus strains was performed, and a 45.6-fold enrichment was achieved at a sorting rate of 300 droplets per second. Furthermore, we used the platform for the selection of higher alpha-amylase-producing Bacillus licheniformis strains from a mutant library generated by atmospheric and room temperature plasma mutagenesis, and clones displaying over 50% improvement in alpha-amylase productivity were isolated. This droplet screening system could be applied to the engineering of other industrially valuable strains.
NSTL
Oxford Univ Press
