Enantioselective analysis of glufosinate using precolumn derivatization with (+)-1-(9-fluorenly)ethyl chloroformate and reversed-phase liquid chromatography

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外文摘要：We have developed a new analytical method to quantify the DL-homoalanine-4-yl(methyl)phosphinate (DL-GLUF) enantiomers in biological specimens using a reversed-phase high-performance liquid chromatography system with a fluorescence detection system. The derivatization of DL-GLUF enantiomers with (+)-1-(9-fluorenyl)ethyl chloroformate was carried out under mild conditions (40 ℃ for 30 min) without inducing racemization. The lower limit of quantitation was 0.01 μg/ml for both D-GLUF and L-GLUF, and the detection limit was 5 ng/ml. When DL-GLUF enantiomers were added to serum to produce concentrations between 0.1 and 100 μg/ml, the mean recovery rate was at least 93.8%. The recovery rate from urine was also satisfactory.

外文关键词：

enantiomer separationderivatizationLCglufosinate

作者：

Yasushi Hori、Manami Fujisawa、Kenji Shimada、Mitsuru Sato、Masao Honda、Yasuo Hirose

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作者单位：

Department of Analytical Chemistry, Niigata College of Pharmacy, Niigata, Japan

Forensic Science Laboratory, Niigata Prefectural Police Headquarters, Niigata, Japan

Emergency and Critical Care Medical Center, Niigata City General Hospital, 2-6-1 Shichkiuyama, Niigata 950-8739, Japan

出版年：

2002

Journal of Chromatography, Biomedical Applications

ISSN：0378-4347

年,卷(期)：2002.776(2)