首页|Production of lactose derivative hetero-oligosaccharides from whey by glucansucrase E81 and determination of prebiotic functions
Production of lactose derivative hetero-oligosaccharides from whey by glucansucrase E81 and determination of prebiotic functions
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NSTL
Elsevier
Bioactive oligosaccharides are important compounds for development of functional foods and ingredients. In this study, lactose derivative hetero-oligosaccharides were produced by acceptor reaction of glucansucrase E81 with whey. The synthesis of oligosaccharides was monitored by Thin Layer Chromatography (TLC) analysis followed by (1 ) and C-13 Nuclear Magnetic Resonance (NMR) spectroscopy analysis which demonstrated the glycosylation of lactose with alpha-(1 -> 2), alpha-(1 -> 3) and alpha-(1 -> 4) linkages. Liquid chromatography-mass spectrometry (LC-MS) analysis demonstrated the formation of DP 7 oligosaccharides from whey. The synthesized oligosaccharides were not digested under in vitro human gastric juice and alpha-amylase conditions. The prebiotic effect of lactose derivative hetem-oligosaccharides was tested and no growth in pathogenic strains was observed whereas certain probiotic strains utilised lactose derivative hetem-oligosaccharides as Carbon source and their growth was promoted. These findings can be important to reveal the potential of glucansucrases to produce functional oligosaccharides from different sources including waste materials.
GlucansucraseOligosaccharidesWheyPrebiotic
Ispirli, Humeyra、Dertli, Enes
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Bayburt Univ, Cent Res Lab, Bayburt, Turkey
Yildiz Tech Univ, Fac Chem & Met Engn, Dept Food Engn, TR-34210 Istanbul, Turkey
NSTL
Elsevier
