首页|Development of a chimeric vaccine candidate based on Toxoplasma gondii major surface antigen 1 and apicoplast proteins using comprehensive immunoinformatics approaches

Development of a chimeric vaccine candidate based on Toxoplasma gondii major surface antigen 1 and apicoplast proteins using comprehensive immunoinformatics approaches

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  • NSTL
  • Elsevier
This study was aimed at designing and evaluation of a multimeric vaccine construct against Toxoplasma gondii via utilization of SAG1 along with apicoplast ribosomal proteins (S2, S5 and L11). Top-ranked MHC-I and MHC-II binding as well as shared, immunodominant linear B-cell epitopes were predicted and joined together via appropriate linkers. Also, TLR-4 agonist (RS-09 synthetic protein) and His-tag were added to the N- and C-terminal of the vaccine sequence. The finally-engineered chimeric vaccine had a length of 291 amino acids with a molecular weight of 31.46 kDa. Physico-chemical features showed a soluble, highly-antigenic and non-allergenic candidate. Secondary and tertiary structures were predicted, and subsequent analyses confirmed the construct stability that was capable to properly interact with human TLR-4. Immunoinformatics-based simulation displayed potent stimulation of T- and B-cell mediated immune responses upon vaccination with the proposed multi-epitope candidate. In conclusion, obtained information demonstrated a highly antigenic vaccine candidate, which could develop high levels of IFN-gamma and other components of cellular immune profile, and can be directed for toxoplasmosis prophylactic purposes.

Chimeric vaccineImmunoinformaticsToxoplasma gondii

Asghari, Ali、Shamsinia, Sadegh、Nourmohammadi, Hassan、Majidiani, Hamidreza、Fatollahzadeh, Mohammad、Nemati, Taher、Irannejad, Hamid、Nouri, Hamid Reza、Ghasemi, Ezatollah、Shams, Morteza

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Shiraz Univ Med Sci, Sch Med, Dept Med Parasitol & Mycol, Shiraz, Iran

Tarbiat Modares Univ, Dept Med Parasitol, Fac Med Sci, Tehran, Iran

Ilam Univ Med Sci, Zoonot Dis Res Ctr, Ilam, Iran

Isfahan Univ Med Sci, Sch Med, Dept Parasitol & Mycol, Esfahan, Iran

Mazandaran Univ Med Sci, Dept Med Chem, Fac Pharm, Sari, Iran

Babol Univ Med Sci, Hlth Res Inst, Cellular & Mol Biol Res Ctr, Babol, Iran

Dezful Univ Med Sci, Sch Med, Dept Med Parasitol, Dezful, Iran

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2021

10.1016/j.ejps.2021.105837

European journal of pharmaceutical sciences

European journal of pharmaceutical sciences

ISTP
ISSN：0928-0987
年,卷(期)：2021.162
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