首页|A two-photon fluorescence probe with endoplasmic reticulum targeting ability for turn-on sensing photosensitized singlet oxygen in living cells and brain tissues
A two-photon fluorescence probe with endoplasmic reticulum targeting ability for turn-on sensing photosensitized singlet oxygen in living cells and brain tissues
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NSTL
Elsevier
Endoplasmic reticulum (ER) is an indispensable organelle responsible for protein synthesis, transportation, and maintenance of Ca2+ homeostasis in eukaryotic cells. Recent studies highlighted that ER-targeted photosensitizers with high yield of singlet oxygen (O-1(2)) are effective in selectively disrupting ER function and are promising candidates for anticancer therapy. Unfortunately, no ER targetable fluorescent probes for determining O-1(2) photosensitized in this photodynamic therapy process is available. In this work, we synthesized an ER-targetable, two-photon fluorescence probe, ER-O-1(2), for fluorescence turn-on sensing of O-1(2). ER-O-1(2) demonstrated high sensitivity to O-1(2) sensing with a wide detection range (0-2.75 mu M) and a low detection limit (0.11 mu M). ER-O-1(2) also displayed excellent selectivity toward O-1(2) out of other ROS and metal ions. Notably, ER-O-1(2) exhibited low cytotoxicity but with specific ER targetable capability. On account of these advantageous features, fluctuations of O-1(2) in living cells and brain tissues were effectively visualized by ER-O-1(2).
NSTL
Elsevier
