首页|Functional expression of the globular domain of human adiponectin in Pichia pastoris.
Functional expression of the globular domain of human adiponectin in Pichia pastoris.
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NSTL
Adiponectin is an adipokine that predominantly synthesized and secreted from adipocytes mainly in the white adipose tissue. Here, we report that we have successfully expressed human gAdiponectin (the globular domain of adiponectin) in the methylotrophic yeast Pichia pastoris after codon optimization and established the purification procedure. The human gAdiponectin gene was designed and synthesized by PCR according to the P. pastoris preferred codons, and then inserted into the P. pastoris pPIC9K expression vector. The plasmid was electroporated into the P. pastoris strain GS115 and only the G418 resistance colonies could produce the gAdiponectin. After fermentation and purification, we could get 1.2g of recombinant gAdiponectin (purity is approximately 95%) from a 24 L culture media. The recombinant gAdiponectin is fully functional as evidenced by induction the phosphorylation of ACC in differentiated C2C12 myotubes, significantly lowering the blood glucose level and accelerating the clearance of freefatty acid in animal models.
HumanadiponectinPichia毕赤酵母
Liu DG、Liu HL、Song TJ、Huang HY、Li X、Tang QQ
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Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical School, Fudan University, PO Box 238, 138 Yi Xue Yuan Road, Shanghai 200032, PR China.
2007
Biochemical and Biophysical Research Communications
NSTL
