首页|Synergistic inhibition of isolated flavonoids from Moringa oleifera leaf on alpha-glucosidase activity
Synergistic inhibition of isolated flavonoids from Moringa oleifera leaf on alpha-glucosidase activity
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NSTL
Elsevier
Flavonoids are phenolic substances naturally distributed in plants with potential antidiabetic activity through the inhibition of alpha-glucosidase. Therefore, the present study investigated the inhibitory effects of alpha-glucosidase activity by purified flavonoids (PF) isolated from Moringa oleifera leaves before and after simulated gastric digestion in the absence or presence of nitrate. Furthermore, the combined effects of PF, chlorogenic acid and acarbose to reduce the acarbose dosage that caused 50% inhibition of alpha-glucosidase activity were studied by using CompuSyn software. Five flavonoids (rutin, kaempferol acetyl glycoside, kaempferol 3-O-glucoside, quercetin-3-glucoside and quercetin-3-acetyl-glucoside) were identified by HPLC-ESI-MS. The results showed that at a concentration of 100 mu g/mL, flavonoids and acarbose inhibited alpha-glucosidase activity by 54.41% and 69.17%, respectively. Lineweaver-Burk plots revealed uncompetitive inhibition of alpha-glucosidase by PF. Flavonoids caused an 8.17 times reduction in acarbose concentration which was required to inhibit alpha-glucosidase. In the absence or presence of nitrate, the inhibitory activity decreased by 48.44% and 72.12%, respectively, after simulated gastric digestion when compared with undigested flavonoids. Rutin and kaempferol 3-O-glucoside showed the highest stability in the simulated gastric liquid, while quercetin-3-acetyl-glucoside and kaempferol 3-O-glucoside exhibited the highest stability in the simulated gastric liquid in the presence of nitrate.
Moringa oleifera leavesFlavonoidsInhibitory activityType II diabetes
Hamed, Yahya Saud、Abdin, Mohamed、Rayan, Ahmed Mohamed、Akhtar, Hafiz Muhammad Saleem、Zeng, Xiaoxiong
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Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
NSTL
Elsevier
