首页|Pectin-associated mannans and xylans play distinct roles in cell-cell adhesion in pine and poplar wood
Pectin-associated mannans and xylans play distinct roles in cell-cell adhesion in pine and poplar wood
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
? 2022 Elsevier B.V.Wood particles of pine required longer treatments of acidic chlorite and dilute alkali to achieve similar proportions of cell separation as of poplar. Chemical extraction and enzymatic digestion studies indicated that rhamnogalacturonan-I (RG-I), mannan and xylan contribute to cell-cell adhesion in pine wood. Mannan associated with lignin in an acidic chlorite fraction and with xylan in a dilute alkali fraction. RG-I and xylan were extracted in both chlorite and dilute alkali in poplar. Scanning electron microscopy and a probe diffusion study using Fluorescence Recovery After Photobleaching showed that lignin, RG-I and mannan initiated cell separation at cell corners and dilute alkali extraction of xylan was required for complete cell separation in pine. Cell-cell separation increased enzymatic saccharification within 24 h by 2-fold in poplar and 3.4-fold in pine. Our results inform a strategy to reduce particle size in woody biomass and provide insights into different molecular bases of cell-cell adhesion.
Liu D.、Yang H.、Carpita N.C.、McCann M.C.、Qian J.、Xu J.
展开 >
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology Department of Food Science and Technology Zhongkai University of Agriculture and Engineering
Key Laboratory of Plant Cell Walls & Plant Resistance Molecular Analysis & Genetic Improvement Center South China Botanical Garden Chinese Academy of Sciences
Department of Botany and Plant Pathology Purdue University
Department of Biological Sciences Purdue University
NSTL
Elsevier
