首页|Evaluating Japanese dace (Tribolodon hakonensis) fish freshness during storage using multispectral images from visible and UV excited fluorescence
Evaluating Japanese dace (Tribolodon hakonensis) fish freshness during storage using multispectral images from visible and UV excited fluorescence
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NSTL
Elsevier
This research aimed at providing a quick, and non-destructive method for estimating freshness of intact Japanese dace (Tribolodon hakonensis) fish refrigerated below 5 degrees C using multispectral imaging technique coupled with multivariate analysis techniques. The fluorescence excitation-emission matrices (EEMs) for outside parts of the fish (eyeball, scales) were taken to establish the proper excitation wavelengths. Afterwards, four lighting devices; white, 395 nm, 365 nm and 280 nm LEDs were used to illuminate the sample for capturing images using both the UV camera and a common color camera. Biochemical analysis (electrophoresis measurement) was carried out to examine fish freshness and then expressed using a reference method as K values. Both EEM and imaging data were modelled with the measured K value using partial least square (PLS) regression. A novel algorithm based on multispectral imaging data was proposed as a potential fish freshness indicator during storage. From the results, R2 of 0.94 and RMSE of 2.42% were achieved. This research demonstrated that the fluorescence multispectral imaging technique is a powerful tool with great potential in non-destructive monitoring and examining fish freshness during storage.
Fish freshnessQuality evaluationExcitation emission matrixUV Excited fluorescenceImage processing
Omwange, Ken Abamba、Saito, Yoshito、Zichen, Huang、Khaliduzzaman, Alin、Kuramoto, Makoto、Ogawa, Yuichi、Kondo, Naoshi、Suzuki, Tetsuhito
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Kyoto Univ, Grad Sch Agr, Lab Biosensing Engn, Sakyo Ku, Kitashirakawa Oiwakecho, Kyoto 6068502, Japan
Ehime Univ, Adv Res Support Ctr, 2-5 Bunkyo Cho, Matsuyama, Ehime 7908577, Japan
NSTL
Elsevier
