首页|THE ROLE OF PYROLIGNEOUS ACID FOR IN VITRO PROPAGATION OF PHORMIUM TENAX 'VARIEGATUM' (J. R. FORST & G. FORST)
THE ROLE OF PYROLIGNEOUS ACID FOR IN VITRO PROPAGATION OF PHORMIUM TENAX 'VARIEGATUM' (J. R. FORST & G. FORST)
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NSTL
The effect of pyroligneous acid (PA), a byproduct of woody biomass pyrolysis, was researched for both antifungal activity and growth promotion in micropropagated in vitro plantlets during the ex vitro acclimatization process. The micropropagated plantlets of Phormium tenax J. R. Forst. & G. Forst 'Variegatum' were transplanted on a coco peat substrate that was treated with PA. The antifungal test was conducted to assess the ability to acclimatization ex vitro. The results showed that the PA completely inhibited soil-borne fungi activity at the concentrations of 1.5, 1.5, and 2.5% of the Rhizoctonia solani (Kuhn), Verticillum dahliae (Kleb.), and Fusarium oxysporum (f. sp. Vasinfectum), respectively. The PA levels were above 86% of the researched Phormium tenax 'Variegatum'. Therefore, both survival rate of acclimatized plantlets and standard coco peat were consistent considering the PA levels. The application of PA into the potting substrate at a rate of 2% and 5% had positive effects on survival rate of >= 90% in terms of plant height, shoot, and root dry biomass, and seedling quality index, compared to the plants acclimatized on coco peat control. The findings demonstrated that the application of PA in potting media at specific concentrations, can suppress soil-borne pathogens by providing plant nutrients. Furthermore, the growth rate promotes and enhance the seedling growth parameters of micro-propagated plants during ex vitro acclimatization process.
NSTL
