首页|Lipidomics study on the molecular changes of eicosapentaenoic and docosahexaenoic acyl structured glycerides during enzyme-catalysis and chemocatalysis
Lipidomics study on the molecular changes of eicosapentaenoic and docosahexaenoic acyl structured glycerides during enzyme-catalysis and chemocatalysis
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NSTL
Elsevier
Enzyme-catalysis and chemocatalysis of fish oil are popularly used in industrial transesterification, but their effect on the intact molecular species is still unclear. This work aimed to trace the variation of lipid molecules and compare the performance of these two methods. During the transesterification reaction, lipidomics study was fully exploited using ultra performance liquid chromatography triple time-of-flight mass spectrometry to characterize the glycerides. The positional distribution of fatty acids in the structured triglycerides was interpreted. A total of 543 glycerides including 15 monoglycerides, 129 diglycerides and 399 triglycerides have been identified and quantified. The results of principal component analysis and orthogonal partial least squares-discriminant analysis displayed a clear molecular phenotype of different catalyzed oils, which also indicated the main difference of the molecules of TG (60:15), TG (60:14), TG (62:16), etc. In conclusion, the enrichment of EPA and DHA by enzyme-catalysis was superior to that by chemical catalysis.
NSTL
Elsevier
