Zootaxa2011,Issue(2748) :11.

Recovery of mitochondrial DNA for systematic studies of Pentatomoidea (Hemiptera: Heteroptera): successful PCR on early 20th century dry museum specimens

JERZY A. LIS DARIUSZ J. ZIAJA PAWEL LIS
Zootaxa2011,Issue(2748) :11.

Recovery of mitochondrial DNA for systematic studies of Pentatomoidea (Hemiptera: Heteroptera): successful PCR on early 20th century dry museum specimens

JERZY A. LIS 1DARIUSZ J. ZIAJA 1PAWEL LIS2
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作者信息

  • 1. Department of Biosystematics, Opole University, Oleska 22, 45-052 Opole, Poland
  • 2. Department of Genetics, Institute of Genetics and Microbiology, University of Wroclaw, S.Przybyszewskiego 63/77, 51-148 Wroclaw,Poland
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Abstract

First molecular studies on museum specimens of five families of pentatomoid bugs, namely Cydnidae, Dinidoridae, Par-astrachiidae, Tessaratomidae, and Thyreocoridae (Hemiptera: Heteroptera: Pentatomoidea), are presented, as a preliminary approach to molecular phylogenetic analyses of these families. Forty-eight pin-mounted museum specimens representing 46 pentatomoid species collected in the late 19lh and the 20th century (more than 15 years old, the oldest specimen collected in 1894) were analyzed; and the acquisition of PCR amplifiable mitochondrial DNA (16S and/or 12S rDNA fragments) was successful from 10 specimens, i.e., 2 specimens (2 species) of Cydnidae, 4 specimens (4 species) of Dinidoridae, 1 specimen (1 species) of Parastrachiidae, 1 specimen (1 species) of Tessaratomidae, and 2 specimens (2 species) of Thyreocoridae. The oldest PCR amplifiable mtDNA sample was extracted from Strombosoma impictum (Stal) (Thyreocoridae) collected in 1932 in Zaire.

Key words

Hemiptera/Heteroptera/Cydnidae/Dinidoridae/Parastrachiidae/Tessaratomidae/Thyreocoridae/ancient DNA/museum specimens/mitochondrial DNA/molecular phylogeny

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出版年

2011
Zootaxa

Zootaxa

SCI
ISSN:1175-5326
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