首页|Application of comparative genomics in the development of DNA probes to detect Bacillus cereus and Bacillus subtilis
Application of comparative genomics in the development of DNA probes to detect Bacillus cereus and Bacillus subtilis
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NSTL
Elsevier
Primer-probe sets to detect Bacillus cereus and/or B. subtilis in food products were developed based on panX (a pan-genome analysis tool). Data for 60 B. cereus strains and 131 B. subtilis strains retrieved from the NCBI RefSeq database were analyzed by panX. Primer-probe sets for B. cereus (ccpA) and B. subtilis (cotQ) were developed by comparing the core genomes of the two bacteria. The developed primer-probe sets' sensitivity and selectivity were identified using 45 strains, including ten B. cereus, five B. subtilis, 13 non-B. cereus and non-B. subtilis strains, and 17 non-Bacillus strains using real-time polymerase chain reaction (PCR). The developed primer-probe sets displayed high sensitivity and selectivity for B. cereus and B. subtilis, validated with two different real-time PCR instruments. PCR analysis using the developed primer-probe showed high efficiency in buffer and milk samples. These results indicate that the primer-probe sets developed based on the panX analysis can be used effectively for detecting B. cereus and/or B. subtilis in food products.
Pan genomeBacillus cereusBacillus subtilisPrimerDetection
NSTL
Elsevier
