首页|Bioengineering of green-synthesized silver nanoparticles: In vitro physicochemical, antibacterial, biofilm inhibitory, anticoagulant, and antioxidant performance

Bioengineering of green-synthesized silver nanoparticles: In vitro physicochemical, antibacterial, biofilm inhibitory, anticoagulant, and antioxidant performance

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  • NSTL
  • Elsevier
Green-synthesized nanobiomaterials can be engineered as smart nanomedicine platforms for diagnostic and therapeutic purposes in medicine. Herein, we investigated the bioengineering of silver nanoparticles (AgNPs) and evaluated their physicochemical, antibacterial, biofilm inhibitory, anticoagulant, and antioxidant perfor-mance. Characterization of the AgNPs was performed utilizing UV-visible, transmission electron microscope (TEM), scanning electron microscope (SEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FT-IR). The spherical shaped AgNPs were proven by TEM and SEM techniques. Moreover, the XRD diffraction patterns demonstrated that the nanoparticles were in a crystalline state. The DLS represented the hydrodynamic particle size of the NPs at 49.62 nm at a pH of 9. The calculated minimum inhibitory concentration (MIC) of AgNPs toward Staphylococcus aureus (ATCC 25923) was 8 mu g mL-1, which was almost similar to tetracycline by the value of 4 mu g mL-1. Moreover, the minimum bactericidal concentration (MBC) of AgNPs was 64 mu g mL-1, which was significantly less than the determined value of 256 mu g mL-1 for tetracycline. Considering the pathogenic and standard S. aureus, the evaluated concentrations of AgNPs and tetracycline showed significant biofilm inhibitory performance. Furthermore, the bioengineered AgNPs exhibited significant anticoagulant activity at 500 mu g mL(-1) compared to saline (P < 0.001). In addition, the biogenic AgNPs inhibited 69.73 +/- 0.56% of DPPH free radicals at 500 mu g mL(-1), indicating considerable antioxidant potential.

Silver nanoparticlesAnticoagulant potentialAntioxidant potentialBiofilm inhibitionAntibacterial performanceSURFACE-PLASMON RESONANCESTAPHYLOCOCCUS-AUREUSMEDIATED SYNTHESISANTIBIOTIC-RESISTANCELEAF EXTRACTOPTIMIZATIONBIOSYNTHESISSHAPESIZEL.

Talank, Niloufar、Morad, Hamed、Barabadi, Hamed、Mojab, Faraz、Amidi, Salimeh、Kobarfard, Farzad、Mahjoub, Mohammad Ali、Jounaki, Kamyar、Mohammadi, Neda、Salehi, Ghazal、Ashrafizadeh, Milad、Mostafavi, Ebrahim

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Univ Mazandaran

Mazandaran Univ Med Sci

Shahid Beheshti Univ Med Sci

Univ Tehran Med Sci

Sabanci Univ

Stanford Univ

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2022

10.1016/j.talanta.2022.123374

Talanta

Talanta

EISCI
ISSN：0039-9140
年,卷(期)：2022.243
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