首页|Ex-vivo mucolytic and anti-inflammatory activity of BromAc in tracheal aspirates from COVID-19
Ex-vivo mucolytic and anti-inflammatory activity of BromAc in tracheal aspirates from COVID-19
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NSTL
COVID-19 is a lethal disease caused by the pandemic SARS-CoV-2， which continues to be a public health threat。 COVID-19 is principally a respiratory disease and is often associated with sputum retention and cytokine storm， for which there are limited therapeutic options。 In this regard， we evaluated the use of BromAc?， a combination of Bromelain and Acetylcysteine (NAC)。 Both drugs present mucolytic effect and have been studied to treat COVID-19。 Therefore， we sought to examine the mucolytic and anti-inflammatory effect of BromAc? in tracheal aspirate samples from critically ill COVID-19 patients requiring mechanical ventilation。 Method: Tracheal aspirate samples from COVID-19 patients were collected following next of kin consent and mucolysis， rheometry and cytokine analysis using Luminex kit was performed。 Results: BromAc? displayed a robust mucolytic effect in a dose dependent manner on COVID-19 sputum ex vivo。 BromAc? showed anti-inflammatory activity， reducing the action of cytokine storm， chemokines including MIP-1alpha， CXCL8， MIP-1b， MCP-1 and IP-10， and regulatory cytokines IL-5， IL-10， IL-13 IL-1Ra and total reduction for IL-9 compared to NAC alone and control。 BromAc? acted on IL-6， demonstrating a reduction in G-CSF and VEGF-D at concentrations of 125 and 250 μg。 Conclusion: These results indicate robust mucolytic and anti-inflammatory effect of BromAc? ex vivo in tracheal aspirates from critically ill COVID-19 patients， indicating its potential to be further assessed as pharmacological treatment for COVID-19。
Patrícia de Melo Oliveira、Daisymara Priscila de Almeida Marques、Linziane Lopes Ferreira、Felipe Alves Clarindo、Murillo Ferreira da Silva、Heitor Portella Povoas Filho、Jordana Grazziela A. Coelho dos Reis、Geovane Marques Ferreira、Alice Aparecida Louren?o、Agata Lopes Ribeiro、Camila Pacheco da Silveira Martins da Mata
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Basic and Applied Virology Laboratory, Department of Microbiology, Institute for Biological
NSTL
