首页|Stereoselective enrichment and determination of citalopram enantiomers by cation-selective exhaustive injection and sweeping coupled with cyclodextrin modified electrokinetic chromatography

Stereoselective enrichment and determination of citalopram enantiomers by cation-selective exhaustive injection and sweeping coupled with cyclodextrin modified electrokinetic chromatography

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  • NSTL
  • Elsevier
An highly sensitive, rapid and enantioselective method was developed for the enantioseparation and determination of citalopram enantiomers by cation selective exhaustive injection-sweeping-cyclodextrin modified electrokinetic chromatography (CSEI-sweeping-CDEKC). The optimized conditions were: 50 mM pH 3.0 phosphate solution with 25 mg.mL(-1) S-beta-CD used as background buffer, 50 mbar 300 s hydrodynamical injection of 150 mM pH 3.0 NaH2PO4 buffer followed with 5 s water plug, 10 kV 600 s electrokinetic sample injection,-20 kV CDEKC run. Under the optimized conditions, the resolution was Rs = 8.04, the enrichment factor as up to 2163 folds, the LOD values were: 3.6 ng.mL(-1) for R-citalopram, 4.1 ng.mL(-1) for S-citalopram, and 3 ng.mL(-1) for both enantiomers in plasma samples. This new method showed good precision, repeatability and stability, which had been successfully applied to the impurity inspection of escitalopram oxalate and the stereoselective pharmacokinetic study of citalopram enantiomers.(c) 2022 Elsevier B.V. All rights reserved.

Citalopram enantiomersCSEI-sweeping-CDEKCEnantioseparationEnantiomer impurity inspectionStereoselective pharmacokinetic studyCAPILLARY-ELECTROPHORESIS METHODSAMPLE STACKINGHUMAN PLASMARACEMIC CITALOPRAMCHIRAL SEPARATIONLC-MS/MSMETABOLITESVALIDATIONDESMETHYLCITALOPRAMPRECONCENTRATION

Zhou, Rui、Wang, Shanshan、Du, Kunze、Shang, Ye、He, Jun、Li, Jin、Yao, Yaqi、Chang, Yan-xu

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Tianjin Univ Tradit Chinese Med

2022

10.1016/j.chroma.2022.462951

Journal of chromatography

Journal of chromatography

ISSN：0021-9673
年,卷(期)：2022.1669
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