首页|Co-overexpression of AVP1, PP2A-C5, and AtCLCc in Arabidopsis thaliana greatly increases tolerance to salt and drought stresses

Co-overexpression of AVP1, PP2A-C5, and AtCLCc in Arabidopsis thaliana greatly increases tolerance to salt and drought stresses

扫码查看
原文链接
  • NSTL
  • Elsevier
Abiotic stresses such as salinity and drought impose a severe constraint on global food production, posing a serious challenge in agriculture. Stacking beneficial genes in transgenic crops will likely improve crop yield under abiotic stress conditions. Previous studies showed that individually overexpressing the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1, the protein phosphatase 2 A catalytic subunit gene PP2A-C5, and the chloride channel protein gene AtCLCc contributed to enhanced salt tolerance and overexpression of AVP1 alone could also improve drought tolerance. We hypothesized that co-overexpressing AVP1, PP2A-C5, and AtCLCc, would combine the benefits of these three genes, leading to a further increase in salt tolerance in transgenic plants due to the potential synergism of these genes. Indeed, co-overexpression of these three genes in Arabi-dopsis significantly improved salt and drought tolerance under single as well as under combined salt and drought stresses. The AVP1/PP2A-C5/AtCLCc co-overexpressing plants displayed robust growth and produced greater amount of biomass as well as viable seeds than wild-type and single gene overexpression plants under saline and drought conditions. This study demonstrates that successful co-overexpression of several well-chosen genes is an effective strategy to achieve greater abiotic stress tolerance and could potentially lead to higher crop yield in regions of the world with saline soil and low precipitation.

Chloride channel proteinDrought stressProtein phosphatase 2APyrophosphataseSalt stressPYROPHOSPHATASE GENE AVP1PROTON-PUMPING PYROPHOSPHATASEPROTEIN PHOSPHATASE 2AH+-PPASEHETEROLOGOUS EXPRESSIONVACUOLAR MEMBRANECHLORIDE CHANNELSANION TRANSPORTERABIOTIC STRESSIMPROVES

Balasubramaniam, Thuvaraki、Wijewardene, Inosha、Hu, Rongbin、Shen, Guoxin、Zhang, Jinlin、Zhang, Hong

展开 >

Texas Tech Univ

Zhejiang Acad Agr Sci

Lanzhou Univ

2022

10.1016/j.envexpbot.2022.104934

Environmental and experimental botany

Environmental and experimental botany

SCI
ISSN：0098-8472
年,卷(期)：2022.200
  • 1
  • 73