首页|Dual-template rectangular nanotube molecularly imprinted polypyrrole for label-free impedimetric sensing of AFP and CEA as lung cancer biomarkers

Dual-template rectangular nanotube molecularly imprinted polypyrrole for label-free impedimetric sensing of AFP and CEA as lung cancer biomarkers

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  • NSTL
  • Elsevier
A high-performance sensing layer based on dual-template molecularly imprinted polymer (DMIP) was fabricated and successfully applied for one-by-one detection of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as lung cancer biomarkers. The plastic antibodies of AFP and CEA were created into the electropolymerized polypyrrole (PPy) on a fluorine-doped tin oxide (FTO) electrode. Raman spectroscopy, field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) tests were performed to pursue the formation and characterization of the sensing layer. Methyl orange (MO) increased the conductivity of PPy and induced the formation of MO doped PPy (PPy-MO) rectangular-shaped nanotubes. Using impedimetric detection, the rebinding of the template antigens was evaluated, the charge transfer resistance increased as the concentration of AFP and CEA increased. The linear dynamic ranges of 5-10(4) and 10-10(4) pg mL(-1) and detection limits of 1.6 and 3.3 pg mL(-1) were obtained for CEA and AFP, respectively. Given satisfactory results in the determination of AFP and CEA in the human serum samples, high sensitivity, and good stability of DMIP sensor made it a promising method for sensing of AFP and CEA in serum samples.

Lung cancer biomarkersImpedimetric sensorMolecularly imprinted polymer (MIP)Alpha-fetoprotein (AFP)Carcinoembryonic antigen (CEA)Electrochemical sensorsCARCINOEMBRYONIC ANTIGENELECTROCHEMICAL SENSORSENSITIVE DETECTIONMETHYL-ORANGEPOLYMERELECTRODEIMMUNOSENSORANTIBODYCARBONSPECTROSCOPY

Taheri, Navid、Khoshsafar, Hosein、Ghanei, Mostafa、Ghazvini, Ali、Bagheri, Hasan

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Baqiyatallah Univ Med Sci

2022

10.1016/j.talanta.2021.123146

Talanta

Talanta

EISCI
ISSN：0039-9140
年,卷(期)：2022.239
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