首页|Lab-In-Syringe automation of deep eutectic solvent-based direct immersion single drop microextraction coupled online to high-performance liquid chromatography for the determination of fluoroquinolones
Lab-In-Syringe automation of deep eutectic solvent-based direct immersion single drop microextraction coupled online to high-performance liquid chromatography for the determination of fluoroquinolones
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
? 2022 Elsevier B.V.Lab-In-Syringe direct immersion single drop microextraction is proposed as an automated sample pretreatment methodology and coupled online to HPLC with fluorescence detection for the determination of fluoroquinolones in environmental waters. For the first time, a drop of a natural deep eutectic solvent (NADES), synthesized from hexanoic acid and thymol, has been used as an extractant in automated single-drop microextraction. The extraction procedure was carried out within the 5 mL void of an automatic syringe pump. A 9-position head valve served the aspiration of all required solutions, air, waste disposal, and hyphenation with the HPLC instrument. Sample mixing during extraction was done by a magnetic stirring bar placed inside the syringe. Only 60 μL of NADES were required omitting toxic classical solvents and improving the greenness of the proposed methodology. By direct injection, linear working ranges between 0.1 and 5 μg L?1 were achieved for all fluoroquinolones. The limit of quantification values and enrichment factors ranged from 20 ng L?1 to 30 ng L?1 and 35 to 45, respectively. Accuracies obtained from the analysis of spiked surface water and wastewater treatment plant effluent analysis at two concentration levels (0.5 and 4 μg L?1) ranged from 84.6% to 119.7%, with RSD values typically <3%.
Automation of sample preparationDirectly immersed single drop microextractionFluoroquinolone antibioticsLab-in-syringeNatural deep eutectic solventOnline coupling to HPLC
Yildirim S.、Solich P.、Horstkotte B.、Cocovi-Solberg D.J.、Uslu B.
展开 >
Karadeniz Technical University Faculty of Pharmacy Department of Analytical Chemistry
Charles University Faculty of Pharmacy Department of Analytical Chemistry
University of Natural Resources and Life Sciences Department of Chemistry Institute of Analytical Chemistry
Ankara University Faculty of Pharmacy Department of Analytical Chemistry
NSTL
Elsevier
