首页|Screening and preservation application of quorum sensing inhibitors of Pseudomonas fluorescens and Shewanella baltica in seafood products

Screening and preservation application of quorum sensing inhibitors of Pseudomonas fluorescens and Shewanella baltica in seafood products

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  • NSTL
  • Elsevier
Pseudomonas fluorescens and Shewanella baltica are specific spoilage organisms (SSOs) of most seafood products. Both of them can cell-to-cell communicate through quorum sensing (QS), facilitating the spoilage activities such as protease secretion, flagellar movement, and biofilm formation. Thus, it is important to screen the quorum sensing inhibitors (QSI) of P. fluorescens and S. baltica. In this study, we tried to apply a high-throughput virtual screening technology to select QSIs candidates. Three amino acid sequences from the key QS regulator-LuxR/I family (the LuxR and LuxI proteins of P. fluorescens, and the LuxR of S. baltica) were targeted and analyzed. Their 3D structures were subsequently constructed for the QSIs candidates' screen of both P. fluorescens and S. baltica. The candidates of cytidine-5'-monophosphate (5'-CMP) and 5'-adenylic acid (5'-AMP) with high scores exerted strong antagonistic effects against both P. fluorescens and S. baltica QS. The addition of 5'-CMP and 5'-AMP also inhibit QS-controlled phenotypes (swimming motility, protease activity, EPS, and biofilm formation) in P. fluorescens and S. baltica. Furthermore, the exogenous addition of 5'-CMP and 5'-AMP could retard the spoilage process of salmon slices during low-temperature storage by inhibiting the production of extracellular protease, siderophores, and Total Volatile Basic Nitrogen (TVB-N).

Pseudomonas fluorescensShewanella balticaVirtual screeningCytidine-5 '-monophosphate5 '-adenylic acid

Wang, Yanbo、Wang, Yongzheng、Chen, Jian、Koseki, Shigenobu、Yang, Qing、Yu, Hejun、Fu, Linglin

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Zhejiang Gongshang Univ, Sch Food Sci & Biotechnol, Food Safety Key Lab Zhejiang Prov, Hangzhou 310018, Peoples R China

Hokkaido Univ, Grad Sch Agr Sci, Kita Ku, Kita 9,Nishi 9, Sapporo, Hokkaido 0608589, Japan

Rongcheng Taixiang Food Prod Co Ltd, Key Lab Frozen Prepared Marine Foods Proc, Taixiang Grp, Minist Agr, Rongcheng, Peoples R China

Zhejiang DaYang Aquat Prod Co Ltd, Zhoushan 316014, Peoples R China

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2021

10.1016/j.lwt.2021.111749

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN：0023-6438
年,卷(期)：2021.149
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