首页|A new near-infrared excitation/emission fluorescent probe for the detection of beta-galactosidase in living cells and in vivo
A new near-infrared excitation/emission fluorescent probe for the detection of beta-galactosidase in living cells and in vivo
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NSTL
Elsevier
Development of noninvasive bioimaging fluorescent probes for detecting particular enzyme activity is greatly recommendable for preclinical diagnosis of cancer. Given that the elevated beta-gal activity is positively correlated with several tumors, developing a fluorescent probe for the sensing of beta-gal is therefore highly desirable for cancer diagnosis. Herein, a new enzyme-activatable near-infrared (NIR) turn-on fluorescent probe (DMC-beta gal) was developed for accurately detecting beta-gal activity characterized by excellent selectivity, high sensitivity (LOD = 0.298 U/L), and low toxicity. More importantly, DMC-beta gal qualifies remarkable NIR excitation (725 nm) and emission wavelength (770 nm), an ideal tool for restrained photodamage and suppressed autofluorescence. The above excellent performance of DMC-beta-gal allowed for the accurate monitoring of endogenous beta-gal in living cells. Moreover, the probe was successfully applied to detect intracellular beta-gal activity in different types of cancer cells, verifying that SKOV-3 cells had a higher level of beta-gal activity than those of A549, HCT-116, MCF-7, and HepG2 cells. Furthermore, DMC-beta gal could real-time visualize endogenously beta-gal in tumor-bearing nude mice with low auto-fluorescence interference. All results fully demonstrated that DMC-beta gal has potential value as a promising strategy for diagnosis of beta-gal-related diseases.
Near-infraredFluorescent probebeta-GalactosidaseCell imagingIn vivo imagingVISUALIZATIONTRACKINGSTRATEGYCYSTEINEDESIGNTUMOR
NSTL
Elsevier
