首页|Real-time PCR identification of Listeria monocytogenes serotype 4c using primers for novel target genes obtained by comparative genomic analysis

Real-time PCR identification of Listeria monocytogenes serotype 4c using primers for novel target genes obtained by comparative genomic analysis

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  • NSTL
  • Elsevier
Detection of the foodborne pathogen Listeria monocytogenes serotype 4c, one of the predominant serotypes in aquatic products, is difficult and time-consuming. Our objective was to identify novel target genes for real-time PCR detection of L. monocytogenes serotype 4c using comparative genomics to facilitate its specific identification in food products. A total of 160 Listeria genome sequences, including those of 12 L. monocytogenes serotypes and 14 other Listeria species, were employed to identify serotype 4c-specific targets. Two serotype 4c-specific genes (SLCC2376_00062 and SLCC2376_00822) were obtained. Using primers for SLCC2376_00822, a real-time PCR assay for the detection of serotype 4c was developed. The specificty of this assay was robustly verified by other serotypes of L. monocytogenes and non-L. monocytogenes strains. In addition, this real-time PCR method could accurately detect serotype 4c even when mixed with different concentrations of serotypes 1/2a and 1/2b. Furthermore, the assay could successfully detect 1.4 x 10(9) -1.4 x 10(3) CFU/g of L. monocytogenes serotype 4c in artificially contaminated grass carp without enrichment. These results demonstrate that the assay, which has high accuracy and sensitivity, comprises a promising approach for the detection and identification of L. monocytogenes serotype 4c strains in foods.

Listeria monocytogenes serotype 4cComparative genomicsNovel molecular targetsReal-time PCR

Li, Fan、Ye, Qinghua、Chen, Moutong、Shang, Yuting、Zhang, Jumei、Ding, Yu、Xue, Liang、Wu, Shi、Wang, Juan、Pang, Rui、Lei, Tao、Zeng, Haiyan、Wu, Qingping

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South China Univ Technol, Sch Biol & Biol Engn, Guangzhou, Peoples R China

Guangdong Acad Sci, Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Safety & Hlth, State Key Lab Appl Microbiol Southern China, Guangzhou, Peoples R China

Jinan Univ, Dept Food Sci & Technol, Guangzhou, Peoples R China

South China Agr Univ, Coll Food Sci, Guangzhou, Peoples R China

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2021

10.1016/j.lwt.2020.110774

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN：0023-6438
年,卷(期)：2021.138
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