首页|Molecular characterization, expression and functional analysis of large yellow croaker (Larimichthys crocea) peroxisome proliferator-activated receptor gamma
Molecular characterization, expression and functional analysis of large yellow croaker (Larimichthys crocea) peroxisome proliferator-activated receptor gamma
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NSTL
Elsevier
The peroxisome proliferator-activated receptor gamma (PPAR gamma) are nuclear receptors with distinct roles in energy metabolism and immunity. Although extensively studied in mammals, immunomodulatory roles of this molecule in teleost fish remain to be investigated. In this study, large yellow croaker (Larimichthys crocea) PPAR gamma (LcPPAR gamma) sequence was cloned, which encodes a polypeptide of 541 amino acids that include signature domains belonging to the nuclear receptor superfamily. Phylogenetically, LcPPAR gamma was most closely related to PPAR gamma derived from European sea bass (Dicentrarchus labrax). Quantitative analysis shown a ubiquitous expression of this molecule, with highest expression level detected in the intestine. The expression of LcPPAR gamma was decreased in the intestine, muscle, body kidney, spleen and head kidney-derived monocytes/macrophages (MO/MCYRILLIC CAPITAL LETTER EFs) over the course of Vibrio alginolyticus (V. alginolyticus) infection. In contrast, an up-regulation of LcPPAR gamma was observed in head kidney-derived MO/MCYRILLIC CAPITAL LETTER EFs following docosahexaenoic acid (DHA) treatment. This increase in LcPPAR gamma leads to an up-regulation of LcCD11b and LcCD18 and an enhancement of complement-mediated phagocytosis. Furthermore, cytokine secretions of V. alginolyticus-stimulated MO/MCYRILLIC CAPITAL LETTER EFs were modulated following LcPPAR gamma activations that up-regulated the expression of LcIL-10, while decreased the expression of LcIL-1 beta, LcTNF-alpha and LcTGF-beta 1. Overall, our results indicated that LcPPAR gamma plays a role in regulating functions of MO/MCYRILLIC CAPITAL LETTER EFs and likely contribute to MO/MCYRILLIC CAPITAL LETTER EFs polarization.
NSTL
Elsevier
