首页|Counter-current fractionation-assisted and bioassay-guided separation of active compounds from cranberry and their interaction with alpha-glucosidase
Counter-current fractionation-assisted and bioassay-guided separation of active compounds from cranberry and their interaction with alpha-glucosidase
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NSTL
Elsevier
An effective method for the selection of active compounds from cranberry based on counter-current fractionation and bioassay-guided separation was established. Cranberry extract showed potential alpha-glucosidase inhibitory activity. The active compounds were extracted by different solvents and enriched in water. The water extract was divided into eight fractions via high-speed counter-current chromatography to further track the active compounds. Results indicated that the alpha-glucosidase inhibitory activity of F7 was remarkable higher than the other fractions. Four compounds, including delphinidin-3-glucoside (D3G), cyanidin-3-glucoside, cyanidin-3-rutinoside, and pelargonidin-3-glucoside, were separated from F7 through column chromatography, and their structures were identified by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry, 1H nuclear magnetic resonance (NMR), and 13C NMR. D3G showed the strongest alpha-glucosidase inhibitory activity among the compounds. Moreover, the interaction mechanism between alpha-glucosidase and D3G was clearly characterized and described by spectroscopic analyses and molecular docking. D3G could spontaneously bind with alpha-glucosidase to form complexes through hydrophobic interaction. The secondary structure of alpha-glucosidase changed in varying degrees after complexation with D3G. The alpha-helix and beta-turn contents of alpha-glucosidase markedly decreased, whereas the irregular coil increased. These findings provide a new insight into the interaction between alpha-glucosidase and D3G.
NSTL
Elsevier
