首页|A water-soluble lysosome-targetable fluorescent probe for carboxylesterase detection and its application in biological imaging
A water-soluble lysosome-targetable fluorescent probe for carboxylesterase detection and its application in biological imaging
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NSTL
Carboxylesterases (CEs) are involved in biotransformation of numerous drugs, environmental toxicants, and carcinogens containing ester groups. Abnormal CEs levels are associated with various diseases. Herein, we developed a novel water-soluble merocyanine-based probe 1 to detect CEs activity using acetate ester as a recognition site. The notable emission at 510 nm of the probe in neat aqueous solution was partially quenched and red-shifted to 580 nm after adding CEs. The fluorescent ratiometric response of 1 toward CEs activity is sensitive (with the detection limit of 0.98 x 10~(-6) U/ml), rapid (within 10 min), and effective in a wide pH range (4.0-10.0). Due to the existence of morpholine as a lysosome-targeting group, the probe was successfully utilized to monitor lysosomal CEs activity. More importantiy, the probe was successfully applied to detect and image carboxylesterase activity in zebrafish.
NSTL
