首页|3-Phenyllactic acid production by Lactobacillus crustorum strains isolated from naturally fermented vegetables
3-Phenyllactic acid production by Lactobacillus crustorum strains isolated from naturally fermented vegetables
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NSTL
Elsevier
3-Phenyllactic acid (3-PLA) is a bioactive compound with broad-spectrum antibacterial and antifungal activity and several desirable pharmacological properties. In this study, 351 lactic acid bacterial strains isolated from naturally fermented Chinese vegetable samples were analyzed for their 3-PLA-producing ability, and three strains showing the greatest 3-PLA-producing ability were selected for further optimizing the fermentation conditions to enhance their 3-PLA yields. The results showed that all the strains were able to produce 3-PLA in MRS broth, but there were considerable variations in their 3-PLA-producing ability among the strains and among the species. Lactobacillus crustorum showed significantly higher 3-PLA-producing ability than the other species. In particular, L. crustorum NWAFU 1078 produced up to 45.2 mmol/L 3-PLA with a 73% substrate conversion rate under the optimized fermentation conditions that the culture was inoculated into MRS broth supplemented with 60 mmol/L phenylpyruvic acid as a substrate, 5.0% CaCO3 as a neutralizer and incubated for 48 h at 30 degrees C. This study indicates the possibility of the biotransformation of phenylpyruvic acid by L. crustorum NWAFU 1078 for highly efficient 3-PLA production.
NSTL
Elsevier
