首页|Spatially hierarchical nano-architecture for real time detection of Interleukin-8 cancer biomarker
Spatially hierarchical nano-architecture for real time detection of Interleukin-8 cancer biomarker
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NSTL
Elsevier
? 2022In the present work we have developed two hierarchical nano-architectures based electrochemical immunosensors for the detection of interleukin-8 (IL-8) cytokine tumor biomarker. A comparative study has been performed for spatial nano-architectures and their relative sensing to establish the model for real time monitoring. With the first platform, the recognition layer consisted with immobilised IL-8 on aminothiol modified gold electrodes. In the second approach, the activated multi walled carbon nanotubes (MWCNT-COOH) were added in the functionalisation process by covalent attachment between the functionalities NH2 of aminothiol and the functionalities COOH of carbon nanotubes. The surface topology of the recognition layer has been characterised by atomic force spectroscopy (AFM) and contact angle (CA) measurements. The electrochemical response of the developed sensor was measured by electrochemical impedance spectroscopy (EIS). A side-by-side comparison showed that aminothiol/activated MWCNTs/anti-IL-8 based impedimetric immunosensor exhibits high reproducibility (The relative standard deviation (R.S.D) = 3.2%, n = 3) with high stability. The present sensor allows evaluating a lower detection limit of 0.1 pg mL?1 with a large dynamic sensitivity range from 1 pg mL?1to 1000 pg mL?1 covering the entire clinical therapeutic window. The developed MWCNTs based immunosensor has been calibrated by determining IL-8 in artificial plasma and showed a selective response to IL-8 even in the interfering environment of other cytokines such as Interleukin-1 (IL-1) and Interleukin-6 (IL-6).
Cancer biomarkerImmunosensorImpedance spectroscopyInterleukin-8Multi wall carbon nanotubes (MWCNT)Spatially hierarchical nano-architecture
Jaffrezic-Renault N.、Errachid A.、Zine N.、Azzouzi S.、Ben Ali M.、Bellagambi F.、Elaissari A.
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Claude Bernard Lyon 1 University (UCBL) Institute of Analytical Sciences (ISA) – UMR 5280
University of Sousse Higher Institute of Applied Sciences and Technology of Sousse GREENS-ISSAT Cité Ettafala
NSTL
Elsevier
