首页|Characterization of protein in cricket (Acheta domesticus), locust (Locusta migratoria), and silk worm pupae (Bombyx mori) insect powders
Characterization of protein in cricket (Acheta domesticus), locust (Locusta migratoria), and silk worm pupae (Bombyx mori) insect powders
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NSTL
Elsevier
Nutritional and functional properties of protein were determined for cricket, locust, and silk worm pupae powders, specifically macro-nutrient proximate composition, full amino acid composition, protein pH-solubility, and SDS-PAGE/densitometry. Cricket and locust powders contained >70 g of crude protein/100 g of sample powder (d.b. - dry basis), while silk worm pupae powder >50 g of crude protein/100 g of sample powder (d.b.). Insect powders exceeded FAO/WHO/UNU recommendations for eight of the nine essential amino acids (EAAs) for adults, but not infants. Total EAAs (21.8-23.7 g EAAs/100 g sample; d.b.) was two-times higher than FAO/ WHO/UNU recommendations for adults (12.7 g EAAs/100 g sample; d.b.), but only half for infants (46.0 g/100 g; d.b.). Nearly 70% of insect protein dissolved in alkaline pH, while only 7% at pH 4-6. SDS-PAGE/densitometry showed four protein fractions: cuticle proteins, actin-arginine kinase, hemocyanin, and myosin. SDS-PAGE/ densitometry also showed proteolysis and various distribution of protein fractions among insect powders. Protein may be efficiently isolated from insects using pH-solubility-precipitation; thus, resulting in isolates with high nutritional and functional quality.
Insect powderInsect proteinProtein propertiesProtein isolateFood product development
Brogan, Emily N.、Park, Yong-Lak、Matak, Kristen E.、Jaczynski, Jacek
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West Virginia Univ, Div Anim & Nutr Sci, POB 6108, Morgantown, WV 26506 USA
NSTL
Elsevier
