首页|Full Paper Galactolipids from Arabidopsis thaliana can replace the function of glucolipids in Bacillus subtilis
Full Paper Galactolipids from Arabidopsis thaliana can replace the function of glucolipids in Bacillus subtilis
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NSTL
Microbiol Res Foundation
Arabidopsis thaliana monogalactosyldiacylglycerol synthase 1(AtMGDl)and digalactosyldiacylglycerol synthase 2(AtDGD2)genes introduced into a Bacillus subtilis chromosome with disrupted galE,which encodes UDP-glucose 4-epimerase,enabled the mutant to produce monogalactosyldiacylglycerol.When galE mutant cells are cultivated in galactose containing medium they show abnormal morphology.This phenotype is correlated with a decrease in the amount of glucolipids.Nucleoids of the ugtP and galE mutants were stained by propidium iodide,which does not permeate intact cell membranes,whereas nucleoids of wild type and of a pssA mutant we examined were not stained.Expression of the AtMGDl gene in a ugtP galE double mutant restored cell membrane integrity.Expression of galactolipid synthase genes from a multi-copy plasmid,pDGHisN4,allowed higher production of galactolipids.Activation of the extracytoplasmic function sigma factors SigM,SigV,and SigX,in the ugtP mutant was decreased by expression of AtMGDl,and SigX activity was strongly repressed when both AtMGDl and AtDGD2 genes were expressed in the mutant.We conclude that the number of sugars that bind to diacylglycerol-rather than the exact sugar species-is important for glycolipid function in B.subtilis.
Department of Biochemistry and Molecular Biology,Graduate School of Science and Engineering,Saitama University,255 Shimo-Okubo,Sakura-Ku,Saitama-Shi,Saitama,338-8570,Japan
NSTL
Microbiol Res Foundation
