首页|First report of <i>Coleus blumei viroid 2</i> from commercial coleus in China.
First report of <i>Coleus blumei viroid 2</i> from commercial coleus in China.
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Amer Phytopathological Soc
In March 2010, leaves were collected from 50 symptomless coleus (<i>Coleus blumei</i>) plants from a commercial nursery in Hainan Province, China. Total RNA was extracted from the leaves. Reverse transcription (RT)-PCR using CbVd-2 specific primers. Amplification of a 301-bp product was obtained from one sample. This PCR product was then cloned into pMD18-T. Twelve positive clones were sequenced and the results were subjected to BLAST analysis. Sequence analysis showed that two sequences (GenBank accessions nos. HQ727542 and HQ727544) shared 99% identity with the reference sequence of CbVd-2 (NC003682), and four sequences (HQ727541, HQ727543, HQ727545 and HQ727547) had 99.34% identity with the reference sequence of CbVd-2 (NC003682). The proposed secondary structures of these variants have approximately 75% paired nucleotides. The results suggested the presence of CbVd-2, which is a member of the Coleviroid genus, Pospoviroidae family. This is thought to be the first report of CbVd-2 from commercial coleus in China.Digital Object Identifier http://dx.doi.org/10.1094/PDIS-12-10-0928
NSTL
Amer Phytopathological Soc
