首页|Fabrication of phospholipid nanofibers containing eugenol@cationic starch nanoparticles against Bacillus cereus in beef
Fabrication of phospholipid nanofibers containing eugenol@cationic starch nanoparticles against Bacillus cereus in beef
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NSTL
Elsevier
A novel controlled-release antibacterial nanofiber was fabricated by electrospinning to load eugenol@cationic starch nanoparticles (EG@CStNPs) into phospholipid nanofibers (EG@CSt/P nanofibers). Taking advantage of the phospholipase and amylase secreted by Bacillus cereus (B. cereus), the controlled release of eugenol in EG@CSt/P nanofibers was achieved through the hydrolysis of the phospholipids in the nanofibers and the cationic starch of the nanoparticles. EG@CStNPs were prepared by nanoprecipitation and characterized by SEM, FTIR, and Raman spectroscopy. The preparation method of phospholipid nanofibers was improved, and nanofibers with enhanced properties were identified through their physical properties. The success of EG@CStNP loading into phospholipid nanofibers was confirmed by FTIR, SEM, AFM and TGA. Subsequently, the success of controlled release was verified by GC-MS, inhibition-zone experiments and TEM. After treatment with EG@CSt/P nanofibers for 5 days, the number of B. cereus decreased by around 2 log(10) reductions at 4 degrees C and 25 degrees C. The results of experiments performed for application in beef further showed that EG@CSt/P nanofibers had significant antibacterial activity against B. cereus and did not damage the color and texture of beef samples.
NSTL
Elsevier
