首页|Structural and interfacial characterization of oil bodies extracted from Camellia oleifera under the neutral and alkaline condition

Structural and interfacial characterization of oil bodies extracted from Camellia oleifera under the neutral and alkaline condition

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  • NSTL
  • Elsevier
Camellia oleifera seed oil contains nearly 80% oleic acid, which accounts for health-benefits, and it is stored in the subcellular oil bodies (OBs) organelles. To utilize C. oleifera OBs as a dietary supplement of unsaturated fatty acid, the physical properties need to be investigated. The physical stabilities of extracted OBs against ionic strength (0.01-1M) and temperature (30-90 degrees C) were evaluated via Turbiscan analysis and confocal microscope. The results showed that OBs extracted at alkaline conditions (pH 9.0-11) exhibited uniform dispersion. The surface charge of the OBs decreased dramatically with increasing NaCl content, and OBs droplet aggregation occurred under a NaCl concentration of 0.1 mol/L. Moreover, high temperature (up to 90 degrees C) had a positive effect on OBs stability. Overall, OBs extracted under alkaline pH were more stable than those extracted under neutral pH. Differences in the interfacial proteins of OBs extracted at the neutral and alkaline pHs were studied via sodium dodecyl sulfate-acrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Six types of oleosins, including Ole III, the fragment of Ole II, oleosin, Ole IV, Ole V, and Ole I, were found in the extracted membrane proteins. Oleosins and some coextracted endogenous proteins played a certain role in OBs stability.

Oil bodiesPhysicochemical propertiesStabilitiesInterfacial proteinsLiquid chromatography-mass spectrometry

Jin, Weiping、Pan, Yijun、Wu, Yi、Chen, Chunling、Xu, Wei、Peng, Dengfeng、Huang, Qingrong

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Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Wuhan, Hubei, Peoples R China

Rutgers State Univ, Food Sci, New Brunswick, NJ USA

Xinyang Normal Univ, Coll Life Sci, Xinyang, Henan, Peoples R China

Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Oilseeds Proc, Minist Agr, Wuhan, Hubei, Peoples R China

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2021

10.1016/j.lwt.2021.110911

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN：0023-6438
年,卷(期)：2021.141
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