首页|Multiplex detection of foodborne pathogens by real-time loop-mediated isothermal amplification on a digital microfluidic chip
Multiplex detection of foodborne pathogens by real-time loop-mediated isothermal amplification on a digital microfluidic chip
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
Rapid detection of foodborne pathogens is critical to assure food quality. In this study, a digital microfluidic (DMF) chip in combination with loop-mediated isothermal amplification (LAMP) was developed for multiplex detection of four common foodborne pathogens. The DMF chip contains ten reaction chambers with dehydrated primers targeting different genes for simultaneous amplification by LAMP in the volume of 2 mu l/reaction with a detection limit of 10(2) CFU/ml for all target bacteria according to the 3s criteria. Using spiked milk for validation, as low as 10(3) CFU/ml of all four pathogenic bacteria were detected simultaneously in a single test within 50 min. The proposed method exhibited higher sensitivity to the commercial benchtop LAMP method with the advantages of simpler operation, high efficiency, and miniaturization, showing great potential for point-of-care (POC) testing of various foodborne pathogens.
Digital microfluidicLoop-mediated isothermal amplificationFoodborne pathogenMultiplex detectionPOLYMERASE-CHAIN-REACTIONCOLORIMETRIC DETECTIONPOINTASSAY
Xie, Mei、Chen, Tianlan、Xin, Xiong、Cai, Zongwei、Dong, Cheng、Lei, Bo
NSTL
Elsevier
