首页|Genetic Engineering of Drought Resistant Potato Plants by Co-Introduction of Genes Encoding Trehalose-6-Phosphate Synthase and Trehalose-6- Phosphate Phosphatase of Zygosaccharomyces rouxii
Genetic Engineering of Drought Resistant Potato Plants by Co-Introduction of Genes Encoding Trehalose-6-Phosphate Synthase and Trehalose-6- Phosphate Phosphatase of Zygosaccharomyces rouxii
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NSTL
Trehalose has been known in many organisms as an energy source and a protectant against several environmental stresses such as freezing, osmotic pressure, heat and desiccation, Trehalose synthesis is mediated by trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) enzyme complex in yeast. In this study, TPS1 and TPS2 genes of Zygosaccharomyces rouvii was engineered under the control of the CaMV 35S promoter along with 2A selfLcleavage sequence for constitutive expressionin transgenic potato plants by Ti-plasmid of Agrobacterium transformation. The resulting construct, ZrTPS2-2A-ZrTPS1, appeared to be transcribed as a single transcript. On the contrary to the previous result that TPS1 transgenic plant showed some morphological alteration, transgenic ZrTPS2-2A-ZrTPS1 potato plant showed no phenotype alteration both in test tube and soil mixture, Most importantly, the transgenic ZrTPS2-2A-ZrTPS1 potato plant showed very strong drought tolerancy We showed the multigene expression system using FMDV 2A sequence could be very useful for two or more gene expression under the control of one promoter and at the same expression level.
NSTL
