首页|Antimicrobial peptide gene expression in Atlantic salmon (Salmo salar) seven days post-challenge with Neoparamoeba perurans
Antimicrobial peptide gene expression in Atlantic salmon (Salmo salar) seven days post-challenge with Neoparamoeba perurans
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
Amoebic gill disease in teleost fish is caused by the marine parasite Neoparamoeba perurans. To date, the role of antimicrobial peptides beta-defensins and cathelicidins in this infection have not been explored. Using a high throughput microfluidics quantitative polymerase chain reaction system (Biomark HD (TM) by Fluidigm), this study aimed to: firstly, to investigate organ-specific expression of antimicrobial peptide genes beta-defensin-1,-3 and-4 and cathelicidin 2 in healthy Atlantic salmon; secondly, to compare the expression of these antimicrobial peptide genes in healthy versus asymptomatic Atlantic salmon seven days post-challenge with Neoparamoeba perurans. Results from this study indicate expression of the beta-defensin and cathelicidin genes in the selected organs from healthy Atlantic salmon. Furthermore, a statistically significant upregulation of beta-defensins-3 and-4 and cathelicidin 2 was detected in gill of parasite-challenged salmon. The upregulated cathelicidin and beta-defensin genes in gill could indicate novel potential roles in innate immune responses to Neoparamoeba perurans.
NSTL
Elsevier
