首页|Upregulation of protein N-glycosylation plays crucial roles in the response of Camellia sinensis leaves to fluoride

Upregulation of protein N-glycosylation plays crucial roles in the response of Camellia sinensis leaves to fluoride

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The tea plant (Camellia sinensis) is one of the three major beverage crops in the world with its leaves consumption as tea. However, it can hyperaccumulate fluoride with about 98% fluoride deposition in the leaves. Our previously studies found that cell wall proteins (CWPs) might play a central role in fluoride accumulation/detoxification in C. sinensis. CWP is known to be glycosylated, however the response of CWP N-glycosylation to fluoride remains unknown in C. sinensis. In this study, a comparative N-glycoproteomic analysis was performed through HILIC enrichment coupled with UPLC-MS/MS based on TMT-labeling approach in C. sinensis leaves. Totally, 237 N-glycoproteins containing 326 unique N-glycosites were identified. 73.4%, 18.6%, 6.3% and 1.7% of these proteins possess 1, 2, 3, and >= 4 modification site, respectively. 93.2% of these proteins were predicted to be localized in the secretory pathway and 78.9% of them were targeted to the cell wall and the plasma membrane. 133 differentially accumulated N-glycosites (DNGSs) on 100 N-glycoproteins (DNGPs) were detected and 85.0% of them exhibited upregulated expression after fluoride treatment. 78.0% DNGPs were extracellular DNGPs, which belonged to CWPs, and 53.0% of them were grouped into protein acting on cell wall polysaccharides, proteases and oxido-reductases, whereas the majority of the remaining DNGPs were mainly related to Nglycoprotein biosynthesis, trafficking and quality control. Our study shed new light on the N-glycoproteome study, and revealed that increased N-glycosylation abundance of CWPs might contribute to fluoride accumulation/detoxification in C. sinensis leave.

Camellia sinensisN -glycoproteinFluorideN -glycoproteomicsPURPLE ACID-PHOSPHATASELINKED GLYCOPROTEOMEMOLECULAR-CLONINGCONCANAVALIN-AHUMAN EXPOSUREARABIDOPSISFAMILYLOCALIZATIONDEGRADATIONPREDICTION

Liu, Yanli、Cao, Dan、Ma, Linlong、Jin, Xiaofang

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Hubei Acad Agr Sci

2022

Plant Physiology and Biochemistry

Plant Physiology and Biochemistry

SCI
ISSN:0981-9428
年,卷(期):2022.183
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