首页|Terpene synthase gene expression evaluation through RT-qPCR in Thymus caespititius
Terpene synthase gene expression evaluation through RT-qPCR in Thymus caespititius
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NSTL
Elsevier
Thymus caespititius Brot. is an interesting aromatic plant due to the valuable monoterpenes it produces considering their properties and biological activities. Several chemotypes have been identified in this species, however, essential oil composition can vary throughout the year in the terpenes' relative percentage. The first objective was to evaluate chemical stability, based on a 20-month survey in essential oil (EO) composition through GC and GC-MS, in two accessions belonging to the carvacrol chemotype, Tc2 and Tc4. Together with this evaluation, a quantitative study of gene expression was performed through reverse transcription quantitative PCR (RT-qPCR), for the most important genes involved in the biosynthesis of gamma-terpinene (precursor of thymol and carvacrol), TcTPS2 and TcTPS4, and alpha-terpineol, TcTPS5. This second objective addressed the correlation between seasonal EO variation and terpene synthase gene expression patterns and compared the trends of seasonal variation between both plant accessions. The results show 22, 48 and 90-fold variation in gene expression for TcTPS2, TcTPS4 and TcTPS5, respectively. Expression of TcTPS5 was much more variable than those coding for gamma-terpinene synthase. The two accessions evaluated showed Tc2 had a more stable gene expression compared to Tc4. Although gene expression and terpene accumulation were in accordance for most timepoints, no significant overall correlation could be found. Our results suggest a transcriptional regulation for TcTPS2 and TcTPS4. For TcTPS5 regulation at post transcriptional level must be present, accounting for differences in the alpha-terpineol relative amount and adding new layers of complexity in specialized metabolism. Further studies should be pursued to identify all the players involved in controlling the final bouquet of essential oil composition.
NSTL
Elsevier
