gamma-aminobutyric acid (GABA) alleviated oxidative damage and programmed cell death in fresh-cut pumpkins

Liang, Jingyi ¹Guo, Fan ¹Cao, Shifeng ²Zhao, Ke ¹Zhao, KeXin ¹Wang, Hongfei ¹Shao, Xingfeng ¹Wei, Yingying ¹Zhang, Chundan ¹Zheng, Yonghua ³Xu, Feng¹

扫码查看

作者信息

1. Ningbo Univ
2. Zhejiang Wanli Univ
3. Nanjing Agr Univ
折叠

Abstract

The regulation of gamma-aminobutyric acid (GABA) on plant anti-oxygenation and programmed cell death (PCD) in fresh-cut pumpkins was investigated. Exogenous GABA positively promoted GABA accumulation and alleviated oxidant damage in pumpkins tissue. Pumpkins treated with GABA showed lower electrolyte leakage, reactive oxygen species (ROS) and MDA content, while higher activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) as compared to the non-treated tissues. Our results also found that GABA treatment retarded DNA degradation and cytochrome c release and delayed the apoptosis of pumpkin cells. On the other hand, the inhibitor of GABA generation, 3-mercaptopropionic acid (3-MP) treatment not only accelerated oxidant damage, but also induced cell death involving chromatin condensation, DNA ladder and cytochrome c releasing. Taken together, our present research indicated that exogenous GABA could alleviate the wound-induced oxidative stress and PCD occurrence in fresh-cut pumpkins.

Key words

Fresh-cut pumpkins/GABA/ROS content/Programmed cell death/BOTRYTIS-CINEREA/PRIMING DEFENSE/REDOX STATUS/SENESCENCE/CALCIUM/ACCUMULATION/METABOLISM/STRESS/FRUIT

引用本文复制引用

出版年

2022

Plant Physiology and Biochemistry

SCI

ISSN：0981-9428

被引量3

参考文献量31

段落导航