首页|Effects of classic antiseizure drugs on seizure activity and anxiety-like behavior in adult zebrafish

Effects of classic antiseizure drugs on seizure activity and anxiety-like behavior in adult zebrafish

扫码查看
原文链接
  • NSTL
  • Elsevier
The zebrafish is extensively used as a model organism for studying several disorders of the central nervous system (CNS), including epilepsy. Some antiseizure drugs (ASDs) have been shown to produce discrepant results in larvae and adults zebrafish, therefore, their anticonvulsant efficacy in subsequent stages of the pentylenetetrazole (PTZ)-induced seizures should be more precisely characterized. The purpose of this study was to investigate behavioral effects of five classic ASDs: valproate (VPA), phenytoin (PHT), carbamazepine (CBZ), diazepam (DZP), and phenobarbital (PB) administered intraperitoneally (i.p.) in the PTZ-induced seizure test in adult zebrafish. We determined the time of maximal effect and the dose-response relationship of the studied ASDs. Furthermore, we assessed changes in the locomotor activity and the anxiety-like behavior in the color preference test. Moreover, drug concentrations in zebrafish homogenates were examined. VPA, DZP, and PB significantly increased the seizure latency at three subsequent stages of seizures (SI?SIII). PHT produced the anticonvulsantlike effect at SI and SII, while CBZ was effective at SII and SIII. Only DZP decreased zebrafish locomotor activity. A strong anxiolytic-like effect was observed after administration of PHT and PB. A weak anxiolytic-like effect occurred after treatment with VPA and DZP. The HPLC analysis showed the average concentrations of the studied ASDs in the fish body during the maximum anticonvulsant activity of each drug. Our results confirm the advantages of using zebrafish with the mature CNS over larval models and its utility to investigate some neuropharmacological properties of the tested drugs.

ZebrafishSeizurePentylenetetrazoleClassic ASDsColor preference

Pierog, Mateusz、Soca, Katarzyna、Doboszewska, Urszula、Wyska, Elzbieta、Guz, Leszek、Szopa, Aleksandra、Serefko, Anna、Poleszak, Ewa、Wlaz, Piotr

展开 >

Marie Curie Sklodowska Univ, Inst Biol Sci, Dept Anim Physiol & Pharmacol, Akad 19, PL-20033 Lublin

Jagiellonian Univ Med Coll, Dept Pharmacokinet & Phys Pharm, Medyczna 9, PL-30688 Krakow, Poland

Univ Life Sci, Inst Biol Bases Anim Dis, Dept Fish Dis & Biol, Akad 12, PL-20033 Lublin, Poland

Med Univ Lublin, Chair & Dept Appl & Social Pharm, Lab Preclin Testing, Chodzki 1, PL-20093 Lublin

展开 >

2021

10.1016/j.taap.2021.115429

Toxicology and Applied Pharmacology

Toxicology and Applied Pharmacology

SCI
ISSN：0041-008X
年,卷(期)：2021.415
  • 8
  • 76