Abstract
Schisandra rubriflora is a dioecious plant of increasing importance due to its lignan composition,and therefore,possible therapeutic properties.The aim of the work was lignan profiling of fruits,leaves and shoots of female(F)and male(M)plants using UHPLC-MS/MS.Additionally,the anti-inflammatory activity of plant extracts and individual lignans was tested in vitro for the inhibition of 15-lipooxygenase(15-LOX),phospholipases A2(sPLA2),cyclooxygenase 1 and 2(COX-1;COX-2)enzyme activities.The extracts of fruits,leaves and shoots of the pharmacopoeial species,S.chinensis,were tested for comparison.Twenty-four lignans were monitored.Lignan contents in S.rubriflora fruit extracts amounted to 1055.65 mg/100 g DW and the dominant compounds included schisanhenol,aneloylgomisin H,schisantherin B,schisandrin A,gomisin O,angeloylgomisin O and gomisin G.The content of lignan in leaf extracts was 853.33(F)and 1106.80(M)mg/100 g DW.Shoot extracts were poorer in lignans-559.97(F)and 384.80(M)mg/100 g DW.Schisantherin B,schisantherin A,6-O-benzoylgomisin O and angeloylgomisin H were the dominant compounds in leaf and shoot extracts.The total content of detected lignans in S.chinensis fruit,leaf and shoot extracts was:1686.95,433.59 and 313.83 mg/100 g DW,respectively.Gomisin N,schisandrin A,schisandrin,gomisin D,schisantherin B,gomisin A,angeloylgomisin H and gomisin J were the dominant lignans in S.chinensis fruit extracts were.The results of anti-inflammatory assays revealed higher activity of S.rubriflora extracts.Individual lignans showed significant inhibitory activity against 15-LOX,COX-1 and COX-2 enzymes.
NSTL
Mdpi