首页|The transcription factor GmbHLH3 confers Cl-/salt tolerance to soybean by upregulating GmCLC1 expression for maintenance of anion homeostasis
The transcription factor GmbHLH3 confers Cl-/salt tolerance to soybean by upregulating GmCLC1 expression for maintenance of anion homeostasis
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
Chloride channels (CLCs)-mediated anion homeostasis plays a crucial role for plants to adapt to Cl-/salt stress; however, the transcriptional regulation of CLCs gene expression in response to Cl-/salt stress is not understood to date. Here, a novel bHLH transcription factor, GmbHLH3, upstream of the GmCLC1 gene, was identified by gene co-expression network analysis. GmbHLH3 localizes to the nucleus and can bind to the G-box at position 550 of GmCLC1 promoter to up-regulate its expression. GmbHLH3-overexpressed soybean hairy root-composite plants and transgenic Arabidopsis exhibited enhanced tolerance to Cl-/salt stress, which was reflected by plant growth phenotype, leaf dead cells, cell membrane damage and lipid peroxidation, and Arabidopsis seed germination. NaCl-treated GmbHLH3-overexpressed soybean plants displayed up-regulated GmCLC1 expression, higher accumulation of Cl- and NO3- in roots for restricting the transport to shoots, and thus maintaining lower Cl-/NO3- ratio in whole plants. Similar effects of GmbHLH3 on the tissue distribution of Cl- and NO3-, and maintenance of anion homeostasis were further verified in transgenic Arabidopsis plants. These findings provide a valuable theoretical basis for further exploration of the transcriptional regulatory molecular networks of plant CLC family members in response to Cl- toxicity under salt stress, as well as seeking molecular genetic improvement approaches for Cl-/salt tolerance in soybean and other crops.
NSTL
Elsevier
