首页|Analysis of cell death in Bacillus subtilis caused by sesquiterpenes from Chrysopogon zizanioides(L.)Roberty

Analysis of cell death in Bacillus subtilis caused by sesquiterpenes from Chrysopogon zizanioides(L.)Roberty

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  • NSTL
  • Microbiol Res Foundation
Recently,the antibacterial effects of essential oils have been investigated in addition to their therapeutic purposes.Owing to their hydrophobic nature,they are thought to perturb the integrity of the bacterial cell membrane,leading to cell death.Against such antibiotic challenges,bacteria develop mechanisms for cell envelope stress responses(CESR).In Bacillus subtilis,a gram-positive sporulating soil bacterium,the extracytoplasmic function(ECF)sigma factor-mediated response system plays a pivotal role in CESR.Among them,σ~M is strongly involved in response to cell envelope stress,including a shortage of available bactoprenol.Vetiver essential oil,a product of Chrysopogon zizanioides(L.)Roberty root,is also known to possess bactericidal activity.oM was exclusively and strongly induced when the cells were exposed to Vetiver extract,and depletion of multi-ECF sigma factors(AsigM,AsigfV,AsigX,and AsigV)enhanced sensitivity to it.From this quadruple mutant strain,the suppressor strains,which restored resistance to the bactericidal activity of Vetiver extract,emerged,although attempts to obtain resistant strains from the wild type did not succeed.Whole-genome resequencing of the suppressor strains and genetic analysis revealed inactivation of xseB orpnpA,which code for exode-oxyribonuclease or polynucleotide phosphorylase,respectively.This allowed the quadruple mutant strain to escape from cell death caused by Vetiver extract.Composition analysis suggested that the sesquiterpene,khusimol,might contribute to the bactericidal activity of the Vetiver extract.

Bacillus subtiliscell deathessential oilssesquiterpenesundecaprenol

Yu Shinjyo、Naoya Midorikawa、Takashi Matsumoto

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Department of Biochemistry and Molecular Biology,Graduate School of Science and Engineering,Saitama University,255 Shimo-Okubo,Sakura-ku,Saitama 338-8570,Japan

Department of Chemistry,Graduate School of Science and Engineering,Saitama University,255 Shimo-Okubo,Sakura-ku,Saitama 338-8570,Japan

Genome Research Center,NODAI Research Institute,Tokyo University of Agriculture,Sakuragaoka 1-1-1,Setagaya-ku,Tokyo 156-8502,Japan

2022

10.2323/jgam.2021.09.005

The Journal of General and Applied Microbiology

The Journal of General and Applied Microbiology

ISSN：0022-1260
年,卷(期)：2022.68(2)
  • 62