首页|Laccase-loaded magnetic dialdehyde inulin nanoparticles as an efficient heterogeneous natural polymer-based biocatalyst for removal and detoxification of ofloxacin
Laccase-loaded magnetic dialdehyde inulin nanoparticles as an efficient heterogeneous natural polymer-based biocatalyst for removal and detoxification of ofloxacin
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Springer Nature
An efficient heterogeneous natural polymer-based biocatalyst was fabricated through the immobilization of laccase onto dialdehyde inulin (DAI)-coated silica-caped magnetic nanoparticles (laccase@DAI@SiO_2@Fe_3O_4?MNPs). The carrier was developed using SiO_2@Fe_3O_4?MNPs and functionalized with DAI. The construction of immobilized laccase was confirmed by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. Immobilization yield and efficiency were calculated as 61.0?±?0.3% and 93.0?±?0.6%, respectively. The immobilized laccase maintained 50% and 85% of its relative activity after 25 repeated cycles and 20 days of storage at 4 °C, respectively. The prepared biocatalyst effectively eliminated ofloxacin, a fluoroquinolone-type antibiotic, with a 63% removal capacity. Besides, antimicrobial activity study on some soil microorganisms involved in the biodegradation of xenobiotics revealed that the laccase-treated ofloxacin resulted in less toxic metabolites. The obtained data indicated that the fabricated biocatalyst is promising for the removal of ofloxacin or other analogs of fluoroquinolones in the environment.
Mahtab Shokri、Somayeh Mojtabavi、Hossein Jafari-Nodoushan、Khashayar Vojdanitalab、Shiva Golshani、Hoda Jahandar、Mohammad Ali Faramarzi
展开 >
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411, Iran
Pharmaceutical Sciences Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
NSTL
Springer Nature
