首页|The underlying mechanisms of anti-hepatitis B effects of formula Le-Cao-Shi and its single herbs by network pharmacology and gut microbiota analysis
The underlying mechanisms of anti-hepatitis B effects of formula Le-Cao-Shi and its single herbs by network pharmacology and gut microbiota analysis
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Formula Le-Cao-Shi (LCS), a traditional Chinese medicine (TCM), has been used as folk remedy for treating hepatitis B for a long time. In our previous study, the anti-hepatitis B effects of LCS have been verified. In the present study, the anti-hepatitis B activities of LCS and its three single herbs were investigated in vitro by HepG2.2.15 cellular model, and the mechanisms against hepatitis B were deciphered via network pharmacology and gut microbiota analysis. By network pharmacology method, twelve key compounds that played a vital role in LCS were filtered from 213 ingredients. The targets RORA, CDK2, RELA, AKT1, IKBKG, PRKCβ and CASP3 were directly related to hepatitis B pathway, which indicated that LCS could exert anti-hepatitis B effect by co-regulating cell cycle and inflammatory pathways. The interactions between candidate compounds and target proteins that were directly involved in hepatitis B pathway were validated by molecular docking simulation and RT-PCR. By gut microbiota analysis, it was revealed that LCS could alter the disordered microbial composition in the infected ducks towards normal, especially the restoration of three key strains, namely Streptococcus alacto-lyticus, Enterococcus cecorum and Bacteroidesfragilis. The above findings could provide a scientific basis for further development and utilization of LCS against hepatitis B.
Traditional Chinese medicineFormula Le-Cao-ShiSingle herbAnti-hepatitis B effectNetwork pharmacologyGut microbiota
Qing Zhao、Shu-Yue Song、Yu-Qi Zhang、Xia Ren、Peng Zhang、Xin Li、Xiu-Mei Fu、Chang-Yun Wang
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Key Laboratory of Marine Drugs, The Ministry of Education of China, School of Medicine and Pharmacy
NSTL
