DNA Sequence-Dependent Binding of Linker Histone gHI Regulates Nucleosome Conformations

扫码查看

原文链接

NSTL

外文摘要：Sequence-dependent binding between DNA and proteins in chromatin is an essential part of gene expression.Linker histone H1 is an important protein in the regulation of chromatin compartmentalization and compaction,and its binding with the nucleosome is sensitive to the DNA sequence.Although the interactions of H1 and DNA have been widely investigated,the mechanism of nucleosome conformation changes induced by the DNA-sequence-dependent binding with gH1(globular H1.0) remains largely unclear at the atomic level.In the present molecular dynamics simulations,both linker and dyad DNAs were mutated to investigate the conformational changes of the nucleosome induced by the sequence-dependent binding of gH1 based on the on-dyad binding mode.Our results indicate that gH1 is insensitive to the DNA sequence of the dyad DNA but presents an apparent preference to linker DNA with an AT-rich sequence.Moreover,this specific binding induces the entry/exit region of a nucleosome to a tight conformation and regulates the accessibility of core histones.Considering that the entry/exit region of the nucleosome is a crucial binding site for many functional proteins related to gene expression,the conformational change at this region could represent an important gene regulation signal.

作者：

Qin Yuan Huo、Yi Qin Gao、Hong Zhang

展开 >

作者单位：

Beijing National Laboratory for Molecular Sciences,College of Chemistry and Molecular Engineering Peking University,Beijing 100871,China

Beijing National Laboratory for Molecular Sciences,College of Chemistry and Molecular Engineering and Biomedical Pioneering Innovation Center(BIOPIC),Peking University,Beijing 100871,China

Beijing National Laboratory for Molecular Sciences,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China

出版年：

2022

DOI：

10.1021/acs.jpcb.2c03785

The journal of physical chemistry

ISSN：1520-6106

年,卷(期)：2022.126(36)

参考文献量58