首页|Ferrearin C induces apoptosis via heme oxygenase-1 (HO-1) induction in neuroblastoma
Ferrearin C induces apoptosis via heme oxygenase-1 (HO-1) induction in neuroblastoma
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
We investigated the cytotoxicity of six neo-lignans (1-6), which are similar in structure to furano-cyclohexenone with an angular allyl group, in human neuroblastoma cell lines (IMR-32, LA-N-1, NB-39, SK-N-SH) and normal cells [human umbilical vein endothelial cells (HUVEC) and human dermal fibroblasts (HDF)] using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Two neolignans, ferrearin C (1) and 2, showed significant cytotoxicity in neuroblastoma cells. Typical morphologic features of apoptosis were observed for the ferrearin-type neolignans using Hoechst 33342, and apoptotic cytoplasmic membrane inversion was also induced by ferrearin-type neolignans in IMR-32. Furthermore, a Proteome Profiler Array showed that the ferrearin-type neolignans induced the marked expression of heme oxygenase-1 (HO-1). In a western blot analysis, ferrearin C (1) increased the level of Bax and reduced the level ofsurvivin, indicating the activation of the mitochondrial pathway of apoptosis.
NeolignanApoptosisNeuroblastomaHeme oxygenase-1Ferrearin C
NSTL
